A Novel Strategy as a Potential Rapid Therapy Modality in the Treatment of Corneal Ulcers: Fluconazole/Vancomycin Dual Drug‐Loaded Nanofibrous Patches

Corneal ulcer, which is brought on by a breach in the epithelial barrier, dangerous infection of avascular corneal stroma. New treatment strategies are needed, suppressing aggressive nature disease and including combination different drugs. In this study, vancomycin (VAN) fluconazole (FLU) dual-drug loaded dual-layered polyvinyl alcohol gelatin (PVA/GEL) nanofibrous patches produced electrospinning. Scanning electron microscopy (SEM) images show smooth surfaces obtained for both pure drug-loaded patches. The tensile test results report that loading FLU VAN separately into PVA/GEL decrease strength elongation at break it further reduced when combining two layers one patch. According to drug release results, VAN-loaded controlled profile extending up 96 h. Moreover, PVA/GEL/FLU, PVA/GEL/VAN, PVA/GEL/FLU/VAN display significant antimicrobial activity against Candida albicans Staphylococcus aureus. SEM, 4'-6diamidynofenyloindol (DAPI) staining, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay PVA/FLU have superior effect NIH3T3 cell spreading proliferation. novelty study lays development potential dual rapid ulcers nature.